Journal: Vaccine

Article Title: Anti-Tumor Effect of the Alphavirus-based Virus-like Particle Vector Expressing Prostate-Specific Antigen in a HLA-DR Transgenic Mouse Model of Prostate Cancer

doi: 10.1016/j.vaccine.2015.08.062

Figure Lengend Snippet: Mice were immunized with VLPV-PSA followed by the inoculation with TRAMP-PSA tumor cells impregnated in Matrigel. Tumor implants were harvested 1, 2 or 3 weeks later (n=3 per treatment group per time point). Cryosections were stained using either goat anti-human PSA (A) or rat anti-mouse CD8 (B) Abs. Images were taken using Zeiss Axioskop microscope with 2.5X objective using AxioVision 3.0 software. Images were scanned using Aperio ImageScope software and Positive Pixel Count Algorithm. Data are expressed as average intensity of staining (positivity) ± SD. Representative IHC images for individual mice are shown in Supplemental Figure 2 (PSA) and Supplemental Figure 3 (CD8). The global effect of the vaccination analyzed by the ANOVA F test was statistically significant for the PSA staining intensity (A) (p=0.0007), although pairwise comparisons between vaccinated and non-vaccinated groups at each time point were only marginally significant (0.05

Article Snippet: To detect PSA, the cryosections were stained with the goat anti-human PSA Ab (R&D Systems) followed by the horseradish peroxidase (HRP)-labeled anti-goat Ig ImmPRESS reagent (Vector Laboratories, Burlingame, CA).

Techniques: Staining, Microscopy, Software